Method of conveniently producing genetically modified non-human mammal with high efficiency
US11470826B2 · kind B2 · utility
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Key dates
| Filing date | Oct 6, 2015 |
| Grant date | Oct 18, 2022 |
| Priority date | — |
| Expiry date | Nov 21, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2330/51
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An object of the present invention is to provide a method of conveniently producing a genetically modified non-human mammal with high efficiency using a CRISPR-Cas9 system and particularly a production method whereby gene knock-in can be achieved with high efficiency regardless of the gene size. The method of producing a genetically modified non-human mammal comprises introducing a Cas9 protein, a crRNA fragment comprising a nucleotide sequence complementary to a target DNA region, and a tracrRNA fragment into a non-human mammalian oocyte to genetically modify the target DNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.