Peronospora resistance in Spinacia oleracea

US11473102B2 · kind B2 · utility

Assignee

• RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V. · NL

Inventors

• Erik den Boer · De Lier, NL
• Raoul Jacobus Johannes Maria Frijters · De Lier, NL

Key dates

• Filing date: Oct 30, 2020
• Grant date: Oct 18, 2022
• Expiry date: Oct 30, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/158
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to an allele designated alpha-WOLF 27 which confers resistance to at least one Peronospora farinosa f. sp. spinacea race, wherein the protein encoded by said allele is a CC-NBS-LRR protein that may comprise in its amino acid sequence: a) the motif “MAEIGYSVC” SEQ ID NO: 1 at its N-terminus; and b) the motif “KWMCLR” SEQ ID NO: 2; and wherein the LRR domain of the protein has in order of increased preference at least 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5%, 99%, 99.5%, 100% sequence similarity to SEQ ID NO: 10. The allele when present in a spinach plant confers complete resistance to at least Peronospora farinosa f. sp. spinacea race Pfs:7, Pfs:8, Pfs:9, Pfs:11, Pfs:12, Pfs:13, Pfs:14, Pfs:15, Pfs:17.