Exopolysaccharide from Rhodopseudomonas palustris and method for preparing and use thereof
US11473115B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Nov 11, 2020 |
| Grant date | Oct 18, 2022 |
| Priority date | — |
| Expiry date | Nov 11, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/01
- WIPO fieldBasic materials chemistry
- WIPO sectorChemistry
Abstract
The invention discloses an exopolysaccharide from Rhodopseudomonas palustris and a method for preparing and use thereof, and the method for preparing comprises the steps of: 1) keeping a seed solution from Rhodopseudomonas palustris GJ-22 in a fermentation medium for fermentation culture to obtain a fermentation broth; 2) centrifuging the fermentation broth to take the supernatant, which is treated by alcohol precipitation after filtration, and then collecting the pellet from alcohol precipitation by centrifugation to obtain crude polysaccharide; 3) removing proteins from the rude polysaccharide using protease enzymolysis method and Sevag method, followed by dialysis treatment with distilled water to remove small molecules and organic solvent to obtain a polysaccharide sample; 4) purifying the polysaccharide sample through an anion exchange column and a molecular exclusion chromatography column obtain the exopolysaccharide from Rhodopseudomonas palustris.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.