Methods, systems, and computer readable media for making base calls in nucleic acid sequencing
US11474070B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 20, 2018 |
| Grant date | Oct 18, 2022 |
| Priority date | — |
| Expiry date | May 20, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B30/20
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for nucleic acid sequencing includes receiving a plurality of observed or measured signals indicative of a parameter observed or measured for a plurality of defined spaces; determining, for at least some of the defined spaces, whether the defined space comprises one or more sample nucleic acids; processing, for at least some of the defined spaces, the observed or measured signal to improve a quality of the observed or measured signal; generating, for at least some of the defined spaces, a set of candidate sequences of bases for the defined space using one or more metrics adapted to associate a score or penalty to the candidate sequences of bases; and selecting the candidate sequence leading to a highest score or a lowest penalty as corresponding to the correct sequence for the one or more sample nucleic acids in the defined space.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.