Digital analysis of molecular analytes using electrical methods
US11474107B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 16, 2022 |
| Grant date | Oct 18, 2022 |
| Priority date | — |
| Expiry date | Mar 16, 2042 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2458/10
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Electrical detection methods are used to identify and further characterize single-molecule target analytes such as proteins and nucleic acids. A composition including a probe region and a tail region is contacted with a target analyte. The probe region specifically binds to the target analyte. The tail region is coupled to the probe region, and includes a nucleic acid template for polynucleotide synthesis. When conditions are such that polynucleotide synthesis occurs along the tail region, one hydrogen ion is released for every nucleotide that is incorporated into the tail region. A transistor such as an ISFET detects and measures changes in ion concentration, and these measurements can be used to identify the tail region and thus characterize the corresponding target analyte.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.