Methods for characterizing and engineering protein-protein interactions

US11474111B2 · kind B2 · utility

Assignee

• A-Alpha Bio, Inc. et al · US

Inventors

• David Younger · Seattle, US
• David Colby · West Lafayette, US
• Randolph Lopez · Seattle, US
• Michael Wittekind · Bainbridge Island, US

Key dates

• Filing date: Dec 16, 2021
• Grant date: Oct 18, 2022
• Expiry date: Dec 16, 2041

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2800/102
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Characterization of the binding dynamics at the interface between any two proteins that specifically interact plays a role in myriad biomedical applications. The methods disclosed herein provide for the high-throughput characterization of the specific interaction at the interface between two protein binding partners and the identification of functionally significant mutations of one or both protein binding partners. For example, the methods disclosed herein may be useful for epitope and paratope mapping of an antibody-antigen pair, which is useful for the discovery and development of novel therapies, vaccines, diagnostics, among other biomedical applications.