Methods for targeted nucleic acid sequence enrichment with applications to error corrected nucleic acid sequencing
US11479807B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 23, 2018 |
| Grant date | Oct 25, 2022 |
| Priority date | — |
| Expiry date | Mar 23, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2535/122
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present technology relates generally to methods and compositions for targeted nucleic acid sequence enrichment, as well as uses of such enrichment for error-corrected nucleic acid sequencing applications. In some embodiments, highly accurate, error corrected and massively parallel sequencing of nucleic acid material is possible using a combination of uniquely labeled strands in a double-stranded nucleic acid complex in such a way that each strand can be informatically related to its complementary strand, but also distinguished from it following sequencing of each strand or an amplified product derived therefrom. In various embodiments, this information can be used for the purpose of error correction of the determined sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.