Method for measuring membrane potential/membrane current of cell
US11480539B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Apr 27, 2018 |
| Grant date | Oct 25, 2022 |
| Priority date | — |
| Expiry date | Aug 9, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/09
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The purpose of the present invention is to provide a method for accurately measuring and controlling intracellular potential by a simple method that is less invasive to the cell and does not require a skilled technique. The present invention makes it possible to provide an intracellular recording electrode inside the cytoplasm by introducing conductive nanoparticles into a cell cultured on a conductive plate electrode, attracting the conductive nanoparticles inside the cell to the side of the cell adhered to the conductive plate electrode, and causing the conductive nanoparticles to pass through the cell membrane. Measuring the current or voltage between the intracellular recording electrode and an extracellular electrode in extracellular solution makes it possible to measure the intracellular potential. In addition, applying a current from one of the electrodes or applying a voltage makes it possible to control the intracellular potential and to measure the activity of the ion channels using a membrane potential fixation method. Similarly, using a magnetic electrode adhered to the cell surface of a target cell into which conductive nanoparticles have been introduced beforehand to …
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.