Enhanced sensitivity and specificity for point-of-care (POC) micro biochip
US11480567B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 15, 2018 |
| Grant date | Oct 25, 2022 |
| Priority date | — |
| Expiry date | Feb 15, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/3276
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An apparatus and method to detect disease-specific antigens assists in disease diagnosis. Point-of-care (POC) micro biochip incorporates at least one hydrophilic microchannel for controlled and self-driven flow of body fluid. Metallic nano-interdigitated electrodes disposed within the channels give enhanced sensitivity detection. Microchannel controls flow and amplifies a capillary effect. Electrodes are fabricated on microchannel surface to detect biomolecular interactions. When a sample flows through microchannel, disease-specific antigens from the sample form antigen-antibody complex with antibodies immobilized on electrodes. Antigen-antibody interaction is detected via an electrical change in the biochip's nano circuit. Each electrode may include a different antibody to detect different antigens. Capacitance during antigen-antibody interaction without microfluidic flow is higher than with microfluidic flow due to immobilized antibodies instability on sensing surface caused by shear stress. POC biochip provides nano level detection of many disease-specific antigens of any type based on micro volume or single drop sized sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.