Virus and antigen purification and conjugation

US11485956B2 · kind B2 · utility

Assignee

• KBIO HOLDINGS LIMITED · GB

Inventors

• Leigh Burden · Owensboro, US
• Steven D. Hume · Owensboro, US
• Joshua D. Morton · Evansville, US
• Greg Pogue · Austin, US
• Barry Bratcher · Owensboro, US
• Hugh A. Haydon · Louisville, US
• Carrie A. Simpson · Evansville, US
• Nick Partain · Owensboro, US
• John W. Shepherd · Owensboro, US

Key dates

• Filing date: Jun 11, 2019
• Grant date: Nov 1, 2022
• Expiry date: Feb 4, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2770/40063
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Disclosed herein are methods and exemplary compositions associated with virus purification, antigen purification, and conjugation of virus and proteins (e.g., antigen) to form vaccines for delivery of immunological and other therapeutic agents, exemplary aspects of which may include harvesting viral and antigenic substances from source organisms; a purification platform comprising chemical separation and size-difference separation for the removal of contaminants, debris and impurities from the viral and protein (e.g. antigenic, including influenza hemagglutinin antigens) substances, as well as their concentration and collection; and a conjugation platform providing activation of the virus at a pH that increases binding rate and binding propensity between the virus and the protein, wherein embodiments related to the conjugation platform include controlling the ratio of virus to protein.