Nucleic acid sequence identification using solid-phase cyclic single base extension
US11485997B2 · kind B2 · utility
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20Claims
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Key dates
| Filing date | Aug 13, 2018 |
| Grant date | Nov 1, 2022 |
| Priority date | — |
| Expiry date | Apr 20, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/166
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present disclosure provides methods, devices and systems for detecting a presence of a nucleic acid molecule having a nucleic acid sequence. Detection of cyclic single base extension can be used to detect a nucleic acid molecule hybridized to a probe and detect a presence of a nucleic acid. The methods disclosed herein can detect a nucleic acid molecule present in a nucleic acid sample at low concentrations and in the presence of background nucleic acids having high sequence similarity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.