In vitro rescue method for immature embryo of avocado

US11490577B2 · kind B2 · utility

Assignees

• HAIKOU EXPERIMENTAL STATION, CHINESE ACADEMY OF TROPICAL AGRICULTRAL SCIENCES · CN
• SANAY RESEARCH INSTITUTE OF CHINESE ACADEMY OF TROPICAL AGRICULTURAL SCIENCES · CN

Inventors

• Jiashui Wang · Zhejiang, CN
• Yanxia Li · Floral Park, US
• Yuanzheng Liu · Haikou, CN
• Weihong Ma · Huizhou, CN
• Xiaoping Zang · Haikou, CN
• He Zhang · Tianjin, CN

Key dates

• Filing date: Mar 30, 2021
• Grant date: Nov 8, 2022
• Expiry date: Mar 30, 2041

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A01H6/00
• WIPO field: Food chemistry
• WIPO sector: Chemistry

Abstract

An in vitro rescue method for immature embryo of avocado includes: collecting immature avocado hybrid fruits, stripping immature zygotic embryos from the sterilized fruits; inducing maturation of zygotic embryos in different media selected according to the maturities of zygotic embryos; and avocado seed embryos growing to normal plants under light conditions after germination. Provided is a technology for the in vitro culture rescue of immature embryo of avocado, wherein the zygotic embryos at different developmental stages are induced to maturation under in vitro conditions, and after maturation, the seed embryos are induced to germinate and form healthy plants. The present invention can effectively improve the survival rate of hybrid avocado zygotic embryo, increase the success rate of hybrid avocado, rescue the hybrid offspring, reduce the loss of hybrid offspring, ensure the genetic integrity of hybrid group, and provide the key technology for hybrid breeding and genetic research of avocado.