Method for measuring protein kinase activity and kit for same
US11492654B2 · kind B2 · utility
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14Claims
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Key dates
| Filing date | May 13, 2014 |
| Grant date | Nov 8, 2022 |
| Priority date | — |
| Expiry date | Apr 6, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/485
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a method of measuring protein kinase activity, including a) attaching GMBS (N-[γ-maleimidobutyryloxy]sulfosuccinimide ester) to a base plate, b) attaching a substrate that reacts with a protein kinase to the base plate having GMBS attached thereto, thus manufacturing a kit for measuring protein kinase activity, c) introducing, to the kit, a mixture of a sample to be analyzed and a buffer including triton X-100, and d) probing phosphorylation of the substrate caused by the protein kinase contained in the sample, thereby measuring the activity of the protein kinase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.