Cell cryopreservation protective composition and use thereof
US11497206B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 7, 2017 |
| Grant date | Nov 15, 2022 |
| Priority date | — |
| Expiry date | Apr 2, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N5/0695
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
The present application relates to a cell cryopreservation protective composition, the use of the composition, and a cell cryopreservation method. The cell cryopreservation protective composition comprises a zwitterionic molecule having the general formula R1—N+(CH3)2—(CH2)n—R2, wherein R1 is a linear or branched alkyl having 1 to 10 carbon atoms, and is optionally substituted with a substituent; R2 is any negatively charged group selected from the group consisting of —COO−, —SO4−, —SO3−, and (I); and R3 is a group selected from the group consisting of (methyl)acryloyloxyalkyl, alkyl and alkenyl; and the zwitterionic molecule having general formula R1—N+(CH3)2—(CH2)n—R2 is preferably a betaine compound. The cell cryopreservation protective composition can carry out cell cryopreservation in a non-toxic and efficient manner, and results in an extremely high post-thaw cell survival rate and does not require stepwise cryopreservation. After cell recovery, the cells can be used directly or after being slightly diluted.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.