Patent · US Active

Plasmid combination and application thereof in preparing modified immune cells

US11497771B2 · kind B2 · utility

0Cited by

0References

17Claims

0Family size

Assignee

Juventas Cell Therapy Ltd. · CN

Inventors

Yun Liu · Nanhu, CN
Lin Shi · Hong Kong, CN
Lulu LV · Tianjin, CN
Pan XIE · Tianjin, CN
Mengmeng CAO · Tianjin, CN
Wang-Yong Yang · Seojong-myeon, KR
Jiaxing Yang · Beijing, CN
Fei Wang · Beijing, CN
Rui Wang · Xiangtan City, CN

Key dates

Filing date	Aug 26, 2021
Grant date	Nov 15, 2022
Priority date	—
Expiry date	Aug 26, 2041

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2740/16043
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

Provided in the present disclosure is a method for using a four-plasmid system to prepare modified immune effector cells. The method comprises: forming a lentivirus by using four plasmids within 293T cells, extracting and obtaining the lentivirus, then transfecting immune effector cells by using the lentivirus, and expressing a chimeric antigen receptor. Also provided in the present disclosure is a use of the immune effector cell obtained by using the described method and of a composition containing the immune effector cell.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.