Methods for scarless introduction of targeted modifications into targeting vectors
US11499164B2 · kind B2 · utility
0Cited by
3References
29Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 30, 2021 |
| Grant date | Nov 15, 2022 |
| Priority date | — |
| Expiry date | Apr 30, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/20
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for introducing a scarless targeted genetic modification into a preexisting targeting vector are provided. The methods can use combinations of bacterial homologous recombination (BHR) and in vitro assembly to introduce such targeted genetic modifications into a preexisting targeting vector in a scarless manner.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.