Analysis of nucleic acids

US11499181B2 · kind B2 · utility

Assignee

• Bio-Rad Laboratories, Inc. · US

Inventors

• John Frederick Regan · San Mateo, US
• Serge Saxonov · Oakland, US
• Michael Lucero · South San Francisco, US
• Benjamin Hindson · Pleasanton, US
• Phillip Belgrader · Severna Park, US
• Simant Dube · Mountain View, US
• Austin So · Pleasanton, US
• Jeffrey Mellen · San Francisco, US
• Nicholas Heredia · Störitzseestraße, US
• Kevin Ness · Pleasanton, US
• Billy W. Colston, Jr. · San Ramon, US

Key dates

• Filing date: Dec 28, 2018
• Grant date: Nov 15, 2022
• Expiry date: Sep 15, 2041

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/172
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Method of haplotype analysis. In an exemplary method, an aqueous phase containing nucleic acid may be partitioned into a plurality of discrete volumes. At least one allele sequence may be amplified in the volumes from each of a first polymorphic locus and a second polymorphic locus that exhibit sequence variation in the nucleic acid. At least one measure of co-amplification of allele sequences from both loci in the same volumes may be determined. A haplotype of the first and second loci may be selected based on the at least one measure of co-amplification.