DNA sequencing by synthesis using modified nucleotides and nanopore detection

US11499186B2 · kind B2 · utility

Assignee

• The Trustees of Columbia University in the City of New York · US

Inventors

• Jingyue Ju · Englewood Cliffs, US
• Shiv Kumar · Montgomery, US
• Zengmin Li · Great Neck, US
• Chuanjuan Tao · Fort Lee, US
• Minchen Chien · Tenafly, US
• James J. Russo · New York, US
• Sergey Kalachikov · New York, US
• Ken Shepard · Ossining, US
• Jacob Rosenstein · Providence, US

Key dates

• Filing date: Oct 15, 2019
• Grant date: Nov 15, 2022
• Expiry date: Dec 19, 2039

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/48721
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

This disclosure is related to a method of sequencing a single-stranded DNA using deoxynucleotide polyphosphate analogues and translocation of tags from incorporated deoxynucleotide polyphosphate analogues through a nanopore.