Acoustic and ultrasound-based mechanogenetics and thermogenetics for immunotherapy

US11504427B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Yingxiao Wang · Village of La Jolla, US
• Shu Chien · Village of La Jolla, US
• Yijia Pan · Village of La Jolla, US
• Yiqian WU · Lanzhou, CN
• Shaoying Lu · Redwood City, US
• Kirk Shung · Monterey Park, US

Key dates

• Filing date: Nov 22, 2017
• Grant date: Nov 22, 2022
• Expiry date: Mar 22, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2830/007
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

In alternative embodiments, provided are compositions, including products of manufacture and kits, and methods, for remotely-controlled and non-invasive manipulation of intracellular nucleic acid expression, genetic processes, function and activity in live cells, e.g., adding functions or changing or adding specificities for immune cells, for monitoring physiologic processes, for the correction of pathological processes and for control of therapeutic outcomes. In alternative embodiments, provided are ultrasound-based thermal or mechanical stimulations, and thermo- or mechano-sensitive protein, either synthetically engineered or natively (endogenously) occurring, integrated to control the production of intracellular nucleic acid and gene expression, e.g., for the expression of biological-active proteins, which can be used, in alternative embodiments, for diagnostic or therapeutic purposes. In alternative embodiments, exemplary thermo- and mechanogenetic systems provided herein allow a deep penetration of stimulation and manipulation in vivo at centimeter-level depth with high spatiotemporal precision.