System and method for isolating and analyzing cells

US11504714B2 · kind B2 · utility

Assignee

• Bio-Rad Laboratories, Inc. · US

Inventors

• Kalyan Handique · Ann Arbor, US
• Vishal Sharma · Kanchinakote, IN
• Priyadarshini Gogoi · Plymouth, US
• William Weiyeh Chow · Los Angeles, US
• Austin Payne · Ann Arbor, US
• Kyle Gleason · Hercules, US
• Brian Boniface · Plymouth, US
• John Connolly · Lynbrook, US
• Sam Tuck · Hercules, US

Key dates

• Filing date: Feb 13, 2020
• Grant date: Nov 22, 2022
• Expiry date: Dec 15, 2040

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2015/1006
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: capturing a population of non-cell particles into the array of wells in single-particle format; releasing, from the non-cell particles, a set of probes into the array of wells; capturing a population of cells into the array of wells in single-cell format; releasing biomolecules from each captured cell into the array of wells; and generating a set of genetic complexes comprising the biomolecules associated with a single captured cell and a subset of probes within individual wells of the array of wells.