Method for improving bio-coupling efficiency between protein and nucleic acid based on alpha-helix handle
US11505800B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 23, 2019 |
| Grant date | Nov 22, 2022 |
| Priority date | — |
| Expiry date | May 10, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/62
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for improving a bio-coupling efficiency between a protein and a nucleic acid based on an α-helix handle includes the following steps. First, the handle carrying the non-natural amino acid (H-tag) is designed. Then, a recombinant expression plasmid encoding a fusion protein containing the H-tag and the protein to be tested is constructed. Subsequently, the fusion recombinant protein containing the non-natural amino acid in the H-tag is expressed and purified. Finally, the non-natural amino acid in the H-tag-fused protein and the coupling group on the nucleic acid substrate are efficiently connected by click chemistry. Thea-helix handle is used to provide a controllable reaction condition on the protein surface for the non-natural amino acid, avoiding the complex structure, charge and polar nanoenvironment around the surface of the protein to be tested.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.