Patent · US Active

Affinity tag for recombination protein recruitment

US11512297B2 · kind B2 · utility

0Cited by
24References
23Claims
0Family size

Assignee

Inventors

Key dates

Filing dateNov 9, 2021
Grant dateNov 29, 2022
Priority date—
Expiry dateNov 9, 2041

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N2800/80
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The present disclosure provides compositions and methods to increase the percentage of edited cells in a cell population when employing nucleic-acid guided editing, as well as automated multi-module instruments for performing these methods. Specifically, the disclosure relates to methods, compositions, modules and automated multi-module cell processing instruments that increase the efficiency of nucleic acid-guided editing in a cell population using a nucleic acid nuclease (i.e., an RNA-guided nuclease or “RGN”)/single-strand binding protein (“SSB”) fusion system. The system leverages a single-strand binding protein (SSP) and single-strand DNA annealing protein (“SSAP”) interactions to drive enhanced recruitment.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.