Methods and compositions for the introduction and regulated expression of genes in plants

US11512321B2 · kind B2 · utility

Assignees

• E.I. DU PONT DE NEMOURS AND COMPANY · US
• PIONEER HI-BRED INTERNATIONAL, INC. · US

Inventors

• William J. Gordon-Kamm · Urbandale, US
• Theodore M. Klein · Wilmington, US
• Keith Lowe · Johnston, US
• Kevin McBride · Davis, US
• Christopher J. Scelonge · Ankeny, US
• Ning Wang · 红钢城街道, CN

Key dates

• Filing date: Feb 22, 2021
• Grant date: Nov 29, 2022
• Expiry date: Jun 6, 2041

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8262
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.