Compositions and methods for detecting pyrophosphate products of enzyme reactions using pyridylazoaniline dyes
US11512342B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | May 4, 2022 |
| Grant date | Nov 29, 2022 |
| Priority date | — |
| Expiry date | May 4, 2042 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/84
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided herein is a composition comprising an enzyme that releases pyrophosphate from a substrate and a dye of Formula 1. A method for detecting pyrophosphate is also provided. A kit comprising a polymerase that releases pyrophosphate by hydrolysis of nucleoside triphosphates during nucleic acid replication, a divalent manganese salt, and the dye are also provided. The present composition, method and kits provide a way to detect and/or quantify substrates or products of enzyme reacted substrates associated with the release pyrophosphate (e.g., nucleic acid amplification reactions and other reactions that hydrolyze ATP) via a distinct color change without substantially affecting the sensitivity and/or specificity of the reaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.