Method and microscopy system for recording an image

US11513328B2 · kind B2 · utility

Assignee

• EUROIMMUN Medizinische Labordiagnostika AG · DE

Inventors

• Helge Pannhoff · Hamburg, DE
• Martin Hagen-Eggert · Stade, DE
• Markus Morrin · Carlow, DE
• Matthias Müller · Kirchheim unter Teck, DE
• Tilman Johannes Sumpf · Hamdorferweide, DE

Key dates

• Filing date: Jun 20, 2018
• Grant date: Nov 29, 2022
• Expiry date: May 28, 2039

Classification

• Technology area (CPC G): Physics
• CPC primary: G02B21/367
• WIPO field: Optics
• WIPO sector: Instruments

Abstract

A method and microscopy system are useful for recording an image of a sample region. A laser beam is directed onto the sample region with interface(s). An objective lens facilitates images the laser beam on a focusing point which lies on the optical axis of the objective lens or an axis parallel thereto, and which lies in a focusing plane. The objective lens and the sample region are displaced with respect to one another in relative fashion along the optical axis of the objective lens to different relative displacement positions. Intensity values of the laser beam are captured for a respective relative displacement position. A respective highest intensity value for a respective displacement position, a curve of the highest intensity values, and a reference relative displacement position from at least one maximum of the curve, are determined. Image(s) of the sample region is/are captured at the reference relative displacement position.