Microfluidic system for sperm separation and enrichment from various types of sperm samples
US11517900B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 29, 2018 |
| Grant date | Dec 6, 2022 |
| Priority date | — |
| Expiry date | Sep 8, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2001/4088
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for separating and enriching sperm from a tissue sample comprises: obtaining a microfluidic separating system having an inlet end and an outlet end, and a membrane filter (e.g., hollow fiber membrane filter) fluidly connected to the outlet end; separating the tissue sample via the microfluidic separating system into a debris fluid volume and a sperm fluid volume; and enriching the sperm fluid volume by removing excess media via the membrane filter. A two-stage tissue sample separation system comprising: a microchannel structure defining a separation fluid channel to form a separation stage; an inlet end of the microchannel structure; an outlet end of the microchannel structure; and a membrane filter fluidly connected to the outlet end for removal of at least a portion of excess media in the tissue sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.