Device and method for automated antibiotic susceptibility testing of gram-negative bacteria

US11518973B2 · kind B2 · utility

Assignee

• Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences · CN

Inventors

• Keming Qu · Beijing, CN
• Xuzhi Zhang · Shandong, CN
• Jun Hong Zhao · Beijing, CN
• Jufa Chen · Shandong, CN
• Yan Zhang · Fort Washington, US
• Qiufen Li · Shandong, CN
• Dongsheng Ding · Shandong, CN
• Xiaochun Wang · Beijing, CN
• Xiaoyu Jiang · Shandong, CN

Key dates

• Filing date: Mar 19, 2019
• Grant date: Dec 6, 2022
• Expiry date: Apr 24, 2039

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/56911
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

An antibiotic susceptibility testing device of gram-negative bacteria, as well as a corresponding method, are discussed. The device has a temperature control unit (including a constant temperature chamber) and a contactless conductivity-based measurement system. Disposable glassy or PVC tubes are used as test vessels for AST. In the performance of AST assay, appropriate kind of liquid medium containing identical amount of target bacterial cells and target antibiotics at different concentrations are loaded into test tubes, following by incubation in the device at a setup temperature. The bacterial growth profile is monitored by collecting the differential values (ΔC) of conductivity of liquid medium, which depend on the proliferation of viable cells. Outcome of ΔC indicates whether the target bacterial cells are completely inhibited by the test antibiotic or not, enabling the user to judge the value of the minimal inhibitory concentration (MIC) simply.