Methods and systems for analyzing nucleic acid molecules

US11519019B2 · kind B2 · utility

Assignee

• Guardant Health, Inc. · US

Inventors

• Andrew KENNEDY · Santa Clara, US
• Stefanie Ann Ward MORTIMER · معلمی نژاد, US
• Helmy A. Eltoukhy · Woodside, US
• AmirAli TALASAZ · Menlo Park, US

Key dates

• Filing date: Jun 24, 2019
• Grant date: Dec 6, 2022
• Expiry date: Jun 24, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/154
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The disclosure provides methods for processing nucleic acid populations containing different forms (e.g., RNA and DNA, single-stranded or double-stranded) and/or extents of modification (e.g., cytosine methylation, association with proteins). These methods accommodate multiple forms and/or modifications of nucleic acid in a sample, such that sequence information can be obtained for multiple forms. The methods also preserve the identity of multiple forms or modified states through processing and analysis, such that analysis of sequence can be combined with epigenetic analysis.