Patent · US Active

Single-cell RNA sequencing using click-chemistry

US11519027B2 · kind B2 · utility

0Cited by

1References

19Claims

0Family size

Assignee

Massachusetts Institute of Technology · US

Inventors

Phillip A. Sharp · Newton, US
Dig Mahat · Somerville, US

Key dates

Filing date	Mar 29, 2019
Grant date	Dec 6, 2022
Priority date	—
Expiry date	Jan 23, 2041

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Y207/01078
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present disclosure relates to a method of sequencing nascent RNA in a cell. In some embodiments, the nascent RNA is conjugated to DNA using copper-catalyzed azide-alkyne cycloaddition (CuAAC). Methods of the present disclosure can be used to generate genomic libraries of a cell and measure gene expression and enhancer and/or super-enhancer activity.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.