Sidwf1 gene controlling internode length in sesame

US11519041B2 · kind B2 · utility

Assignee

• Henan Sesame Research Center, Henan Academy Of Agricultural Sciences · CN

Inventors

• Haiyang Zhang · Shanghai, CN
• HONGMEI MIAO · Zhengzhou, CN
• Chun Li · Beijing, CN
• Yinghui DUAN · Zhengzhou, CN
• Libin Wei · Zhengzhou, CN
• MING JU · Zhengzhou, CN

Key dates

• Filing date: Jul 22, 2020
• Grant date: Dec 6, 2022
• Expiry date: Jul 22, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/156
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A Sidwf1 gene of Sesamum indicum, including two exons and an intron, is 1638 bp in total, and has a sequence represented by SEQ ID NO: 1. Also provided is a method for determining the internode length type in sesame samples, the method including: 1) extracting a genomic DNA of a sesame sample; 2) synthesizing three primers including SiSNPdwf1 F1, SiSNPdwf1 F2, and SiSNPdwf1 R; amplifying the Sidwf1 gene or an allele SiDWF1 thereof with the genomic DNA of the sesame sample as a template, with a combination of SiSNPdwf1 F1, SiSNPdwf1 F2, and SiSNPdwf1 R a combination of SiSNPdwf1 F1 and SiSNPdwf1 R, or a combination of SiSNPdwf1 F2 and SiSNPdwf1 R, as primers, thereby yielding a PCR product; and performing electrophoresis on the PCR product or sequencing the PCR product, and determining the phenotype of the sesame sample according to an electrophoresis or sequencing result.