Ultrasound-assisted simulated digestion method of milk protein active peptide and application thereof in health foods
US11524977B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 12, 2018 |
| Grant date | Dec 13, 2022 |
| Priority date | — |
| Expiry date | Jun 1, 2039 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA23V2002/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An ultrasound-assisted simulated digestion method of a milk protein active peptide and an application thereof in health foods, pertaining to the technical field of intensive processing of dairy products and preparation of health foods. The method firstly employs ultrasonic pretreatment of casein and β-lactoglobulin, followed by enzymatic hydrolysis with a protease to prepare casein and ρ-lactoglobulin polypeptide, and traces the activity of the polypeptide by simulating gastrointestinal digestion, and then simulates absorption by intestinal epithelial cells with Caco-2 cells, to characterize a highly active milk protein polypeptide digested by the gastrointestinal tract and absorbed by the Caco-2 cells simulating absorption by the inner wall of the small intestine. The method has identified five such highly active milk protein polypeptides.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.