Engineered spider silk proteins and uses thereof

US11524984B2 · kind B2 · utility

Assignee

• SPIBER TECHNOLOGIES AB · SE

Inventors

• Anna Rising · Uppsala, SE
• Jan Johansson · Stockholm, SE
• Marlene Andersson · Uppsala, SE

Key dates

• Filing date: Jun 29, 2017
• Grant date: Dec 13, 2022
• Expiry date: Dec 10, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2533/50
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A recombinant spider silk protein, consisting of no more than 800 amino acids, comprising a set of domains arranged according to the formula (NT)-REP-CT, wherein: the optional NT-domain, if present, comprises a sequence of 100 to 160 amino-acid residues derived from the N-terminal domain of a spider silk protein; the REP-domain comprises a sequence of 30 to 600 amino acid residues derived from the repetitive segment of a spider silk protein; and the CT-domain comprises a sequence of 70 to 120 amino acid residues derived from the C-terminal domain of a spider silk protein selected from: a sequence of 72 to 110 amino acid residues derived from the C-terminal domain of a spider silk protein, wherein the sequence comprises at least 7 residues independently selected from K, R, E and D; a sequence having at least 85% identity to SEQ ID NO: 15 or any one of SEQ ID NOs: 62-65 or 67-73; and a sequence having at least 70% identity to SEQ ID NOs: 64 or any one of SEQ ID NOs: 62-65 or 67-73, wherein the sequence comprises at least 7 residues independently selected from K, R, E and D.