Process for the isolation and analysis of microorganisms contained in a sample
US11525115B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 8, 2019 |
| Grant date | Dec 13, 2022 |
| Priority date | — |
| Expiry date | May 13, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/26
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A process is provided for isolating and analyzing microorganisms contained in a sample by collecting a determined volume in a sample, the determined volume representing all or part of this sample, likely to contain at least one microorganism. The collected volume is then split up into a plurality of compartments having a culture medium, the volume of each compartment being smaller than 10 μL, each compartment being isolated from the other compartments and having no interface with the ambient atmosphere. At least one microorganism is incubated in the compartments for determined durations, and compartments are detected that contain at least one microorganism. The incubation may be extended after detecting compartments so that at least one microorganism having a defined quantity can be detected, and then the content of the detected compartments can be recovered. Finally, at least one functional parameter relative to a microorganism in the compartments is determined.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.