Methods for simultaneous amplification of target loci

US11525162B2 · kind B2 · utility

Assignee

• Natera, Inc. · US

Inventors

• Matthew Rabinowitz · San Francisco, US
• Matthew Hill · Belmont, US
• Bernhard Zimmermann · Manteca, US
• Johan Baner · San Francisco, US
• George Gemelos · Walnut, US
• Milena Banjevic · Los Altos Hills, US
• Allison Ryan · Redwood City, US
• Styrmir Sigurjonsson · San Jose, US
• Zachary Demko · San Francisco, US

Key dates

• Filing date: Mar 25, 2020
• Grant date: Dec 13, 2022
• Expiry date: Mar 25, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/156
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.