Method for screening of tobacco nicotine converters

US11525814B2 · kind B2 · utility

Assignee

• Yunnan Academy of Tobacco Agricultural Sciences · CN

Inventors

• Yong Li · Corvallis, US
• Tao Pang · 安丰镇, CN
• Xuejun Chen · Zhuhai, CN
• Xueyi Sui · Kunming, CN
• Junli SHI · Kunming, CN
• Yongping Li · Kunming, CN
• Guanghui Kong · Kunming, CN
• Ping Chen · Nanhu, CN
• Yuping Wu · Kowloon, CN

Key dates

• Filing date: Dec 29, 2020
• Grant date: Dec 13, 2022
• Expiry date: Jun 19, 2041

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2030/8886
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method for identifying converters from tobacco seedling population. The method includes: 1) sowing and cultivating tobacco seeds to be identified for 45-55 days; sampling a plurality of leaf disks from each of 45-55 days old seedlings; 2) incubating the plurality of leaf disks of each seedling in a sealed container at 37° C. for 10-12 hours, thereby obtaining a plurality of incubated tobacco leaves of each seedling; 3) immersing the plurality of incubated tobacco leaves of each seedling in an extractant, extracting alkaloids and obtaining an extract of each seedling; 4) analyzing the amounts of nicotine and nornicotine in the alkaloids extract of each seedling; and 5) automatically recognizing peaks of the alkaloids extract of each seedling, and calculating the percent nicotine conversion (PNC) and the pseudo percent nicotine conversion (PPNC).