Nanostraw well insert devices for improved cell transfection and viability

US11530378B2 · kind B2 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Ryan T. SWOBODA · Orange Park, US
• Yuhong Cao · Palo Alto, US
• Sergio Leal-Ortiz · Hayward, US
• Stefanie Rothkoetter · Stanford, US
• Nicholas A. Melosh · Menlo Park, US

Key dates

• Filing date: Jun 9, 2017
• Grant date: Dec 20, 2022
• Expiry date: Apr 19, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2539/00
• WIPO field: Surface technology, coating
• WIPO sector: Chemistry

Abstract

Described herein are nanostraw well insert apparatuses (e.g., devices and systems) that include nanotubes extending through and out of a membrane so that a material can pass through the membrane from a fluid reservoir depot and into a cell grown onto the nanotubes when electrical energy (e.g., electroporation energy) is applied. In particular, the device, systems and methods described herein may be adapted for cell growth viability and transfection efficiency (e.g., >70%). These apparatuses may be readily integratable into cell culturing processes for improved transfection efficiency, intracellular transport, and cell viability.