DPO4 polymerase variants with improved accuracy
US11530392B2 · kind B2 · utility
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Key dates
| Filing date | Dec 10, 2018 |
| Grant date | Dec 20, 2022 |
| Priority date | — |
| Expiry date | Dec 10, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y207/07007
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Recombinant DPO4-type DNA polymerase variants with amino acid substitutions that confer modified properties upon the polymerase for improved single molecule sequencing applications are provided. Such properties may include enhanced binding and accurate incorporation of bulky nucleotide analog substrates into daughter strands and the like. Also provided are compositions comprising such DPO4 variants and nucleotide analogs, as well as nucleic acids which encode the polymerases with the aforementioned phenotypes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.