Rapid and efficient de-glycosylation of glycoproteins

US11530403B2 · kind B2 · utility

Assignee

• Biocon Ltd. · IN

Inventors

• Anushikha Thakur · Vittasandra, IN
• Seija Rohil · Yadiyuru, IN
• Shrivardhan Patil · Kolhapur, IN
• Meenakshi Sudhakaran · Kochi, IN
• Laxmi Adhikary · Savanuru, IN

Key dates

• Filing date: Nov 17, 2017
• Grant date: Dec 20, 2022
• Expiry date: Nov 17, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2317/41
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention discloses rapid and cost-effective method of de-glycosyation of a glycoprotein, wherein, glycoprotein is combined with anionic surfactant and reducing agent and non-ionic surfactant in order to obtain stable denatured glycoprotein. An endoglycosidase is further added to denatured glycoprotein to cleave N-linked glycans in order to obtain de-glycosylated protein. A rapid tool for assessing the protein conformation by partial de-glycosylation is also presented wherein the partial de-glycosylated protein is analysed using capillary electrophoresis (CE-SDS).