Prunus persica polygalacturonase-inhibiting protein 1 (PpPGIP1) gene, and cloning method and use thereof
US11530416B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 13, 2021 |
| Grant date | Dec 20, 2022 |
| Priority date | — |
| Expiry date | Dec 13, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8205
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention discloses a Prunus persica polygalacturonase-inhibiting protein 1 (PpPGIP1) gene, and a cloning method and use thereof. The Prunus persica polygalacturonase-inhibiting protein 1 PpPGIP1 gene has a nucleotide sequence shown in SEQ ID NO: 1, and a protein encoded by the Prunus persica polygalacturonase-inhibiting protein 1 (PpPGIP1) gene has an amino acid sequence shown in SEQ ID NO: 2. The cloning method includes the following steps: (1) extracting total RNA from a peach, and subjecting the total RNA to reverse transcription to obtain cDNA, which serves as a template; (2) designing primers based on the PpPGIP1 gene sequence; and (3) Polymerase Chain Reaction (PCR) amplification: conducting PCR amplification to obtain a PpPGIP1 gene amplification product.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.