Highly specific circular proximity ligation assay
US11530438B2 · kind B2 · utility
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Key dates
| Filing date | Feb 20, 2018 |
| Grant date | Dec 20, 2022 |
| Priority date | — |
| Expiry date | Feb 10, 2039 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/542
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided herein is a circular proximity ligation assay in which proximity-probes are employed as bridges to connect two free oligonucleotides via a dual ligation event, resulting in the formation of a circle. The circles are then quantified by, e.g., qPCR. The addition of an extra oligonucleotide is believed to enhance specificity by decreasing the probability of random background ligation events. In addition, circle formation may have selective advantages, as uncircularized DNA can be removed by a simple exonuclease treatment and it has streamlined the workflow by eliminating preamplification prior to qPCR.
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