Non-destructive bilayer monitoring using measurement of bilayer response to electrical stimulus

US11530443B2 · kind B2 · utility

Assignee

• Roche Sequencing Solutions, Inc. · US

Inventors

• Kevin Aliado · Sunnyvale, US
• Roger Chen · Saratoga, US
• Jing Luo · Sacramento, US
• William Nielsen · Aiea, US
• Kyle Umeda · Saratoga, US
• Ashraf Wahba · Hayward, US

Key dates

• Filing date: Jun 3, 2020
• Grant date: Dec 20, 2022
• Expiry date: Oct 5, 2040

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/48721
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.