Vaccine used for preventing toxoplasma gondii infection and preparation method therefor

US11534485B2 · kind B2 · utility

Assignee

• Haimu Animal Health Products (Shandong) Co., Ltd.

Inventors

• Feng Hou · Beijing, CN
• Lili Cao · Palomar Court, CN
• Pengtao Gong · Palomar Court, CN
• Siming Li · Palomar Court, CN
• Xingyuan Chen · Palomar Court, CN
• He DING · Palomar Court, CN
• Dian Wang · Beijing, CN

Key dates

• Filing date: Oct 22, 2018
• Grant date: Dec 27, 2022
• Expiry date: Oct 22, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Provided is a protein having Toxoplasma immunogenicity, the protein being a cyclophilin mutant protein and consisting of the amino acid sequence as shown in SED 2. Further provided is a nucleic acid that may encode a protein having Toxoplasma immunogenicity, which has the nucleic acid sequence as shown in SEQ ID NO. 1. Further provided is a vaccine, which is obtained by double-digesting a Toxoplasma antigen gene and then linking the same to a prokaryotic expression vector such as pET28a, and transforming the same into a prokaryotic expression engineering strain such as BL21(DE3), thereby inducing the high-efficiency expression thereof, wherein the inducing the high-efficiency expression thereof, wherein the purified protein is a soluble protein which maintains specific immunogenicity thereof.