Bivalent bispecific antibody hybrid protein expression and preparation methods
US11535674B2 · kind B2 · utility
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10Claims
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Key dates
| Filing date | Dec 16, 2016 |
| Grant date | Dec 27, 2022 |
| Priority date | — |
| Expiry date | Dec 22, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/92
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure discloses a method for expressing and preparing a bivalent bispecific antibody. In the present disclosure, each portion of a bivalent bispecific antibody and an immune hybrid protein thereof is respectively expressed in a suitable prokaryotic or eukaryotic cell system, separated and purified by high-performance affinity chromatography, and then spliced in vitro by trans-splicing reaction mediated by an intein, to prepare the bivalent specific antibody and an immune hybrid protein thereof.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.