Methods for improving the health of porcine species by targeted inactivation of CD163

US11535850B2 · kind B2 · utility

Assignee

• Genus plc · US

Inventors

• Andrew Mark Cigan · Madison, US
• Jonathan Lightner · Johnston, US
• Matthew Scott Culbertson · Wilmington, US
• William Thomas Christianson · Hendersonville, US
• Benjamin Beaton · DeForest, US
• Brian Burger · Madison, US
• Dylan Barnes · Madison, US
• Matthew Campbell · Dublin, IE

Key dates

• Filing date: Jan 21, 2022
• Grant date: Dec 27, 2022
• Expiry date: Jan 21, 2042

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/124
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure relates methods and compositions useful for prevention of porcine reproductive and respiratory syndrome virus (PRRSv) in animals, including animals of the species Sus scrofa. The present teachings relate to swine wherein at least one allele of a CD163 gene has been inactivated, and to specific methods and nucleic acid sequences used in gene editing to inactivate the CD163 gene. Swine wherein both alleles of the CD163 gene are inactivated are resistant to porcine reproductive and respiratory syndrome virus (PRRSv). Elite lines comprising homozygous CD163 edited genes retain their superior properties.