Use of transposase and Y adapters to fragment and tag DNA
US11535889B2 · kind B2 · utility
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13Claims
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Key dates
| Filing date | Mar 7, 2019 |
| Grant date | Dec 27, 2022 |
| Priority date | — |
| Expiry date | Oct 27, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6874
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Described herein is an adapter comprising a population of first oligonucleotides, a second oligonucleotide and a third oligonucleotide, wherein the first oligonucleotides, the second oligonucleotide and the third oligonucleotide are hybridized together to produce a complex that comprises: (i) a first end comprising a transposase recognition sequence, (ii) a central single-stranded region of variable sequence and (iii) a second end comprising sequences that are non-complementary. A method, as well as a kit for practicing the method, are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.