Patent · US Active

Mass spectrometry assay method for detection and quantitation of liver function metabolites

US11536726B2 · kind B2 · utility

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13Claims
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Key dates

Filing dateApr 1, 2019
Grant dateDec 27, 2022
Priority date
Expiry dateSep 23, 2039

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG01N2800/085
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

A method for determining in a sample, by mass spectrometry, the amount of one or more analytes selected from the group consisting of 12,13-DiHOME, 3-hydroxybutyrate (BHBA), 3-hydroxyoctanoate, 3-methylglutarylcarnitine, 3-ureidopropionate, 7-alpha-hydroxy-4-cholesten-3-one (7-Hoca), citrate, fucose, fumarate, gamma-tocopherol, glutamate, glutarate, glycerol, glycochenodeoxycholate, glycocholate, hypoxanthine, maleate, malonate, mannose, orotate, 2,3-pyrdinedicarboxylate, ribose, serine, taurine, taurochenodeoxycholate, taurocholate, palmitoleate, linolenate, xanthine, xylitol, and combinations thereof is described. The method comprises subjecting the sample to an ionization source under conditions suitable to produce one or more ions detectable by mass spectrometry from each of the one or more analytes; measuring, by mass spectrometry, the amount of the one or more ions from each of the one or more analytes; and using the measured amount to determine the amount of each of the one or more analytes in the sample.

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