Systems and methods for automated cell segmentation and labeling in immunofluorescence microscopy
US11538261B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 12, 2019 |
| Grant date | Dec 27, 2022 |
| Priority date | — |
| Expiry date | Dec 12, 2039 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG06T2207/30024
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
Various techniques are provided for performing automated full-cell segmentation and labeling in immunofluorescent microscopy. These techniques perform membrane segmentation and nuclear seed detection separate and independently from each other, then combine their results to identify cell boundaries. Some embodiments use texture- and kernel-based image processing to perform the method. In some embodiments, the method for obtaining membrane features disclosed herein can be used in conjunction with or separate from the nuclear features. The results can be used for a variety of purposes, including whole-area cell segmentation in fluorescence-based tissue imaging.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.