Patent · US Active

Targeted gene disruption methods and immunogenic compositions

US11541108B2 · kind B2 · utility

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23Claims
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Key dates

Filing dateApr 30, 2018
Grant dateJan 3, 2023
Priority date—
Expiry dateOct 4, 2038

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY02A50/30
  • WIPO fieldPharmaceuticals
  • WIPO sectorChemistry

Abstract

Targeted disruption of a specific gene and its subsequent restoration in obligate intracellular bacteria remains extremely challenging due to their absolute requirement for residence inside a host cell to replicate. Here, targeted allelic exchange mutations were created to inactivate two genes and then to restore one of the two genes of a rickettsial pathogen, Ehrlichia chaffeensis. These methods were then also successfully utilized in Ehrlichia canis and Anaplasma phagocyophilum. The resultant mutated pathogens are useful in immunogenic compositions for reducing the incidence of or severity of infection with ricksettsial pathogens.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.