Group of UDP-glycosyltransferase for catalyzing carbohydrate chain elongation and application thereof

US11542484B2 · kind B2 · utility

Assignee

• CAS CENTER FOR EXCELLENCE IN MOLECULAR PLANT SCIENCES · CN

Inventors

• Zhihua Zhou · Shanghai, CN
• Wei Wei · Lo Wu, CN
• Xing Yan · Aberdeen, GB
• Chengshuai Yang · Shanghai, CN
• Chaojing Li · Shanghai, CN
• Yongjun Wei · Hangzhou City, CN
• Pingping Wang · Shanghai, CN

Key dates

• Filing date: May 21, 2018
• Grant date: Jan 3, 2023
• Expiry date: Aug 20, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P33/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a group of glycosyltransferase, and an application thereof. Specifically, provided is using glycosyltransferase GT29-32, GT29-33, GT29-34, GT29-4, GT29-5, GT29-7, GT29-9, GT29-11, GT29-13, GT29-17, GT29-18, GT29-19, GT29-20, GT29-21, GT29-22, GT29-23, GT29-24, GT29-25, GT29-36, GT29-37, GT29-42, GT29-43, GT29-45, GT29-46, PNUGT29-1, PNUGT29-2, PNUGT29-3, PNUGT29-4, PNUGT29-5, PNUGT29-6, PNUGT29-7, PNUGT29-8, PNUGT29-9, PNUGT29-14, and PNUGT29-15, as well as derived polypeptides thereof to catalyze the first glycosyl at position C-20, the first glycosyl at position C-6, and the first glycosyl at position C-3 of a tetracyclic triterpene compound substrate to elongate a carbohydrate chain, thereby obtaining a catalytic reaction of ginsenoside products such as ginsenoside Rg3, ginsenoside Rd, ginsenoside Rb1, ginsenoside Rb3, saponin DMGG, saponin DMGX, gypenoside LXXV, gypenoside XVII, gypenoside XIII, gypenoside IX, notoginsenoside U, and notoginsenoside R1, notoginsenoside R2, notoginsenoside R3, 3-O-β-(D-xylopyranosyl)-β-(D-glucopyranosyl)-PPD, 3-O-β-(D-xylopyranosyl)-β-(D-glucopyranosyl)-CK, 20-O-Glucosylginsenoside Rf, and Ginsenoside F3. Glycosyl…