Microarray based multiplex pathogen analysis and uses thereof
US11542498B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 8, 2018 |
| Grant date | Jan 3, 2023 |
| Priority date | — |
| Expiry date | Dec 13, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/5308
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided herein is a 3-dimensional lattice microarray system for DNA sequence detection and analysis. The system has a plurality of bifunctional polymer linkers, on one end of which are attached nucleic acid probes where each have a sequence complementary to signature nucleotide sequences in pathogens, plants or animals. The other end of the bifunctional polymer linker is attached to a solid support by non-covalent or covalent means. Each of the nucleic acid probes have terminal thymidine bases at the 5′ and 3′ ends that permit attachment of the probes to the bifunctional polymer linkers. Also provided is a method for fabricating the microarray system by first attaching the bifunctional polymer linkers to the solid support, followed by photochemical coupling of the nucleic probes to the microarray. A customizable microarray kit is provided that contains the solid support, linkers, probes, solvent mixture and instructions to use the kit.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.